The Extraction and analysis of Glycerides by GCMS and IR in Oil seeds from the Sesbania sesban plant in Manipur
Keywords:
: Chuchurangmei, Sesbania sesban, transesterification, non-edible vegetable oil, Biodiesel.Abstract
The oil obtained from Sesbania sesban seeds was extracted using a solvent extraction method with petroleum ether as an extracting solvent. To ensure purity for subsequent analysis, the oil underwent a purification process using column chromatography with a silica gel (60-120 mesh) column and an eluent composed of a mixture of petroleum ether and ethyl acetate (20:1 ratio). After this purification, the oil was subjected to transesterification, resulting in the production of biodiesel also referred to as fatty acid methyl ester (FAME).
The composition of fatty acid methyl esters (FAME) in Sesbania sesban seed oil was determined through NMR, IR and GCMS analysis. The fame profile of Sesbania sesban oil comprises of 5.21wt.% of methyl palmitate [C16:0], 93.17 wt.% of methyl linoleate [C18:2] and 1.61 wt.% of methyl stearate [C18:0].
